| # | Athlete | Yr. | Team | Seed | Note | |
|---|---|---|---|---|---|---|
| 1 | 155 | FAN, Fiona | Brown University Running Club | 26.50 | ||
| 2 | 666 | FOTINO, Rose | SR | Tufts University Running Club | 26.68 | 2024 Mini Meet at Boston University |
| 3 | 470 | STEPANENKO, Lydia | UMass Amherst Club Running | 27.00 | ||
| 4 | 214 | BAKER, Jane | Brown University Running Club | 29.10 | ||
| 5 | 339 | TERRANOVA, Ella | PC Running Club | 29.50 | ||
| 6 | 602 | GILL, Abigail | SR | URI Club Track and Field | 29.51 | Bruno's Backyard Classic 2024 |
| 7 | 10 | COSTELLO, Madelyn | Quinnipiac Running Club | 30.00 | ||
| 8 | 334 | REILING, Lauren | PC Running Club | 30.00 | ||
| 9 | 599 | ELIZONDO, Emma | JR | URI Club Track and Field | 32.20 | |
| 10 | 24 | LEIMGRUBER, Grace | Quinnipiac Running Club | 36.00 | ||
| 11 | 91 | GLOWACKI, Jessica | UConn Running Club | 40.00 | ||
| 12 | 630 | BERDINKA, Molly | URI Club Track and Field | NT |